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phosphorylated bcl 2 associated death promoter p bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated bcl 2 associated death promoter p bad
    Phosphorylated Bcl 2 Associated Death Promoter P Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+bad+ser136/Phospho-Bad+(Ser136)+Rabbit+mAb/pm40112539-103-24-30
    Average 95 stars, based on 131 article reviews
    phosphorylated bcl 2 associated death promoter p bad - by Bioz Stars, 2026-11
    95/100 stars

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    Incubation:

    Article Title: PROM2 promotes gemcitabine chemoresistance via activating the Akt signaling pathway in pancreatic cancer
    Article Snippet: Cell lysates were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). .. The membranes were incubated with antibodies against PROM2 (1:500, Abcam, Cambridge, MA, USA), p-Akt (Ser473) (1:2000, Cell Signaling Technology, Danvers, MA, USA), Akt1 (1:1000, Cell Signaling Technology), p-BAD (Ser136) (1:500, Cell Signaling Technology), BAD (1:1000, Cell Signaling Technology), p-Caspase-9 (Ser 196) (1:500, Abcam), Caspase-9 (1:1000, Abcam), Flag (1:1000, Sigma, St Louis, MO, USA) and HA (1:500, Sigma) overnight at 4 °C, then incubated with horseradish peroxidase-conjugated secondary antibodies (Goat anti-rabbit/mouse, PIERCE, Waltham, MA, USA) for 1 h at room temperature. .. The blotting membranes were stripped and re-probed with an anti-α-Tubulin antibody (1:1000, Sigma).

    Isolation:

    Article Title: Sweroside attenuates podocyte injury and proteinuria in part by activating Akt/BAD signaling in mice.
    Article Snippet: Funding information Science and Technology Plan Project of Huaihua, Grant/Award Number: 2021R3103; Hunan Provincial Natural Science Foundation of China, Grant/Award Number: 2023JJ50434; Scientific Research Fund of Hunan Provincial Education Department, Grant/Award Number: 22A0705; Doctoral Scientific Research Foundation of Hunan University of Medicine, Grant/Award Number: 2020122001 Abstract In this study, we investigated the effects of sweroside on podocyte injury in diabetic nephropathy (DN) mice and elucidated its molecular mechanisms.. We conducted in vivo experiments using a C57BL/6 mice model of DN to explore the effects of sweroside on proteinuria and podocyte injury in DN mice.. In in vitro experiments, conditionally immortalized mouse podocytes were treated with high glucose and sweroside, and the protective effects of sweroside on podocyte injury were analyzed.

    other:

    Article Title: ROR1 Regulates Chemoresistance in Triple Negative Breast Cancer and Can Be Targeted with Strictinin for Selective Anti-Cancer Therapy
    Article Snippet: BT—549 cells were cultured in RPMI (Lonza, Basel, Switzerland), with FBS (10%), insulin (’lOug/ml), and penicillin (100 U/ml) and streptomycin (0.1 mg/ml).



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    phosphorylated bcl 2 associated death promoter p bad  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc phosphorylated bcl 2 associated death promoter p bad
    Phosphorylated Bcl 2 Associated Death Promoter P Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+bad+ser136/Phospho-Bad+(Ser136)+Rabbit+mAb/pm40112539-103-24-30
    Average 95 stars, based on 1 article reviews
    phosphorylated bcl 2 associated death promoter p bad - by Bioz Stars, 2026-11
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    cell death p bad  (Cell Signaling Technology Inc)
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    Cell Death P Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p bad ser136  (Cell Signaling Technology Inc)
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    Figure 4. PGG inhibits ROR1 intracellular domain phosphorylation, ROR1 expression, down- stream oncogenic signaling, and anti-apoptotic signaling in PC3 cells. (A) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (B) Quantification of immunoblot bands phospho-ROR1 (Tyr786) and ROR1, all nor- malized to GAPDH. Data are expressed as the mean +/−SEM from three independent western blots. (C) Representative western blot of phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (D) Quantification of im- munoblot bands phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β, all normalized to GAPDH. (E) Representative western blot of apoptotic markers XIAP, phospho-Bad <t>(ser136),</t> and Bad after treatment of PC3 cells with vehicle or 31.25 µM PGG for 24 h. (F) Quantification of immunoblot bands XIAP, phospho-Bad (ser136), and Bad, all normalized to GAPDH. Data are ex- pressed as the mean +/−SEM from three independent western blots. [N = 3. *** = p value < 0.001; * = p value < 0.05; ns = not significant].
    P Bad Ser136, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p bad ps136  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc p bad ps136
    Figure 4. PGG inhibits ROR1 intracellular domain phosphorylation, ROR1 expression, down- stream oncogenic signaling, and anti-apoptotic signaling in PC3 cells. (A) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (B) Quantification of immunoblot bands phospho-ROR1 (Tyr786) and ROR1, all nor- malized to GAPDH. Data are expressed as the mean +/−SEM from three independent western blots. (C) Representative western blot of phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (D) Quantification of im- munoblot bands phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β, all normalized to GAPDH. (E) Representative western blot of apoptotic markers XIAP, phospho-Bad <t>(ser136),</t> and Bad after treatment of PC3 cells with vehicle or 31.25 µM PGG for 24 h. (F) Quantification of immunoblot bands XIAP, phospho-Bad (ser136), and Bad, all normalized to GAPDH. Data are ex- pressed as the mean +/−SEM from three independent western blots. [N = 3. *** = p value < 0.001; * = p value < 0.05; ns = not significant].
    P Bad Ps136, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+bad+ser136/Phospho-Bad+(Ser136)+Rabbit+mAb/10__1158_slash_2767___9764__crc___23___0308-64-80-82
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    p bad  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc p bad
    Figure 4. PGG inhibits ROR1 intracellular domain phosphorylation, ROR1 expression, down- stream oncogenic signaling, and anti-apoptotic signaling in PC3 cells. (A) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (B) Quantification of immunoblot bands phospho-ROR1 (Tyr786) and ROR1, all nor- malized to GAPDH. Data are expressed as the mean +/−SEM from three independent western blots. (C) Representative western blot of phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (D) Quantification of im- munoblot bands phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β, all normalized to GAPDH. (E) Representative western blot of apoptotic markers XIAP, phospho-Bad <t>(ser136),</t> and Bad after treatment of PC3 cells with vehicle or 31.25 µM PGG for 24 h. (F) Quantification of immunoblot bands XIAP, phospho-Bad (ser136), and Bad, all normalized to GAPDH. Data are ex- pressed as the mean +/−SEM from three independent western blots. [N = 3. *** = p value < 0.001; * = p value < 0.05; ns = not significant].
    P Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Figure 4. PGG inhibits ROR1 intracellular domain phosphorylation, ROR1 expression, down- stream oncogenic signaling, and anti-apoptotic signaling in PC3 cells. (A) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (B) Quantification of immunoblot bands phospho-ROR1 (Tyr786) and ROR1, all nor- malized to GAPDH. Data are expressed as the mean +/−SEM from three independent western blots. (C) Representative western blot of phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (D) Quantification of im- munoblot bands phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β, all normalized to GAPDH. (E) Representative western blot of apoptotic markers XIAP, phospho-Bad (ser136), and Bad after treatment of PC3 cells with vehicle or 31.25 µM PGG for 24 h. (F) Quantification of immunoblot bands XIAP, phospho-Bad (ser136), and Bad, all normalized to GAPDH. Data are ex- pressed as the mean +/−SEM from three independent western blots. [N = 3. *** = p value < 0.001; * = p value < 0.05; ns = not significant].

    Journal: International journal of molecular sciences

    Article Title: Pentagalloyl Glucose (PGG) Exhibits Anti-Cancer Activity against Aggressive Prostate Cancer by Modulating the ROR1 Mediated AKT-GSK3β Pathway.

    doi: 10.3390/ijms25137003

    Figure Lengend Snippet: Figure 4. PGG inhibits ROR1 intracellular domain phosphorylation, ROR1 expression, down- stream oncogenic signaling, and anti-apoptotic signaling in PC3 cells. (A) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (B) Quantification of immunoblot bands phospho-ROR1 (Tyr786) and ROR1, all nor- malized to GAPDH. Data are expressed as the mean +/−SEM from three independent western blots. (C) Representative western blot of phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β on PC3 cells treated with vehicle or 31.25 µM PGG for 24 h. (D) Quantification of im- munoblot bands phospho-AKT (ser473), phospho-GSK3β (ser9), AKT, and GSK3β, all normalized to GAPDH. (E) Representative western blot of apoptotic markers XIAP, phospho-Bad (ser136), and Bad after treatment of PC3 cells with vehicle or 31.25 µM PGG for 24 h. (F) Quantification of immunoblot bands XIAP, phospho-Bad (ser136), and Bad, all normalized to GAPDH. Data are ex- pressed as the mean +/−SEM from three independent western blots. [N = 3. *** = p value < 0.001; * = p value < 0.05; ns = not significant].

    Article Snippet: 2024, 25, 7003 18 of 24 XIAP (Cell signaling, USA, #14334S); Bad (Cell signaling, USA, #9239S); p-Bad-ser136 (Cell signaling, USA, #4366S); Snail (Cell signaling, USA, #3895S); Twist1 (Cell signaling, USA, #69366S); MMP-9 (Cell signaling, USA, #13667S); Cyclin A2 (Cell signaling, USA, #4656S); Cyclin D1 (Cell signaling, USA, #2978S); GAPDH (scbt, Santa Cruz, CA, USA, #C2819); HSP40 (Cell signaling, USA, #4868S); IRDye 800CW Goat anti-rabbit secondary (Licor, Lincoln, NE, USA, #926-32211); IRDye 800CW Goat anti-mouse secondary (Licor, USA, #926-32210); HRP conjugated Goat anti-mouse secondary (Biorad, Hercules, CA, USA, #1706516); HRP conjugated Goat anti-rabbit secondary (Cell signaling, USA, #7074S).

    Techniques: Phospho-proteomics, Expressing, Western Blot